Verified Document

Structure Of Jann 2411 Article Review

¶ … structure of Jann_2411( DUF14790) from Jannaschia sp at 1.45 A resolution reveals a new fold ( the ABATE domain) and suggests its possible role as transcription regulator" The purpose of this study was to resolve the structure of the protein Jann_2411 and relate its structure to the functional properties of the protein. The researchers determined the structure using the method of x-ray crystallography (multiple wavelength anomalous diffraction) and structural analysis of the protein revealed it to be a putative stress-related transcription factor.

Jann_2411 has a molecular weight of 20.7 kDa (residues 1-187) and a calculated isoelectric point of 6.6 and its crystal structure was determined using the semiautomated high-throughput pipeline of the Joint Center for Structural Genomics. Structural analysis revealed a two domain organization with the N-terminal domain consisting of a new fold called ABATE (Alpha-beta hairpin and Alpha TandEm) domain and the C-terminal domain forming a treble-clef zinc finger after a characteristic conserved sequence. Jan_2411 forms a dimer that binds DNA forming a putative stress-related transcription factor for which the ABATE domain is implicated.

However, the structure shows that Jann_2411 is actually comprised of two domains. The first domain (residues 1 -- 142) can be visualized as two subdomains (H2 -- H4, 1 -- 2 and H5 -- H7, 3 -- 4) that share similar topology and secondary-structure elements, namely a helix -- -hairpin -- helix motif (H2-1-2-H3 in the first subdomain; H6-3- 4-H7 in the second subdomain), with an additional helix (H4 from the first subdomain and H5 from the second subdomain) linking the two motifs. The researchers have therefore named this region the ABATE domain, representing the Alpha-Beta-hairpin-Alpha TandEm motif.
The second domain (residues 143 -- 187; H8, 5 -- 6, H9) forms a treble-clef zinc finger. The zinc ion is coordinated by two cysteines (Cys147 and Cys152) from a loop termed the zinc knuckle, located between the strands of the third -hairpin (5 -- 6), and two cysteines from the N-terminus of helix H9 (Cys168 and Cys172). This arrangement of zinc-coordinating residues is typical of treble-clef zinc fingers. Other strictly conserved residues in this domain include Asp158 and Arg175. A…

Sources used in this document:
Works Cited

Bakolitsa C. et al. (2009) The structure of Jann_2411(DUF14790) from Jannaschia sp at 1.45 A resolution reveals a new fold ( the ABATE domain) and suggests its possible role as transcription regulator. Structural Biology and Communications.
Cite this Document:
Copy Bibliography Citation

Related Documents

Biochemistry of Hnrna C. And
Words: 8906 Length: 24 Document Type: Thesis

123). In this study, Martinez-Contreras and her associates report the results of recent research that has provided additional evidence concerning the function of these proteins in precursor-messenger RNA (pre-mRNA) splicing (2007). The splicing repression can function in two discrete ways in heterogeneous nuclear RNP proteins; the first way is by antagonizing the recognition of splice sites directly and the second way is through interference with the binding of proteins that

Biochemistry Similarity of Glycolysis in Prokaryotes and
Words: 519 Length: 2 Document Type: Essay

Biochemistry Similarity of Glycolysis in Prokaryotes and Eukaryotes Glycolysis is reported as being a pathway that is practically universal for energy extraction that carbohydrates hold available and this is true for eukaryotes, prokaryotes as well as aerobes and anaerobes. (Essential Biochemistry, 2014, paraphrased) Only eukaryotes have mitochondria. Some prokaryotes are reported to be photosynthetic and to use "an electron transport chain to make ATP." (Essential Biochemistry, 2014, p. 1) It is believed that

Biochemistry the Polypeptide Chain Configuration in Hemoglobin
Words: 411 Length: 1 Document Type: Article Review

Biochemistry The Polypeptide Chain Configuration in Hemoglobin and other Globular Proteins By Linus Pauling and Robert B. Corey (1951) The article named above describes a continuation in the study of hydrogenbonded planar-amide configurations of polypeptide chains. It also discusses evidence on the possibility of the existence of such chains in fibrous proteins, and in molecules of globular proteins. The article first notes that globular patterns such as ovalbumin, can "on denaturation be converted into

Biochemistry the Effect of BRCA1
Words: 1553 Length: 5 Document Type: Term Paper

Altogether, the Icelandic findings implicate epigenetic silencing of the BRCA1 gene in sporadic breast tumors. They even suggest that "the BRCA1 gene is implicated in sporadic breast tumorigenesis through epigenetic silencing and deletion of the BRCA1 gene," and that BRCA1 methylation is also found to be important in hereditary breast cancer, indicating similarities between BRCA1 methylated and hereditary BRCA1 breast tumors (Birgisdotter, et al. 9). While heredity factors strongly contribute

Database Search for Biochemistry Breakthroughs
Words: 664 Length: 2 Document Type: Essay

The next database used for the search is Medline. I began with the same search terms, celiac disease biochemistry, however it did not return any articles. Next, the search was expanded my search by simply searching celiac disease. This returned 544 articles. The first article is titled Celiac Disease and is from the National Library of Medicine. This article is clearly not useful. Next the search was expanded to celiac

Patent Law
Words: 678 Length: 2 Document Type: Creative Writing

student with a Bachelor of Science degree in Communications, minors in both Psychology and Sociology, and a desire to attend law school, my request to enter a Biochemistry program may be unusual. After all, people who focus on the hard sciences usually do so because of plans to work in a particular industry or to pursue additional field-specific education via Masters or PhD programs. Because I have no intention

Sign Up for Unlimited Study Help

Our semester plans gives you unlimited, unrestricted access to our entire library of resources —writing tools, guides, example essays, tutorials, class notes, and more.

Get Started Now