¶ … structure of Jann_2411( DUF14790) from Jannaschia sp at 1.45 A resolution reveals a new fold ( the ABATE domain) and suggests its possible role as transcription regulator"
The purpose of this study was to resolve the structure of the protein Jann_2411 and relate its structure to the functional properties of the protein. The researchers determined the structure using the method of x-ray crystallography (multiple wavelength anomalous diffraction) and structural analysis of the protein revealed it to be a putative stress-related transcription factor.
Jann_2411 has a molecular weight of 20.7 kDa (residues 1-187) and a calculated isoelectric point of 6.6 and its crystal structure was determined using the semiautomated high-throughput pipeline of the Joint Center for Structural Genomics. Structural analysis revealed a two domain organization with the N-terminal domain consisting of a new fold called ABATE (Alpha-beta hairpin and Alpha TandEm) domain and the C-terminal domain forming a treble-clef zinc finger after a characteristic conserved sequence. Jan_2411 forms a dimer that binds DNA forming a putative stress-related transcription factor for which the ABATE domain is implicated.
The second domain (residues 143 -- 187; H8, 5 -- 6, H9) forms a treble-clef zinc finger. The zinc ion is coordinated by two cysteines (Cys147 and Cys152) from a loop termed the zinc knuckle, located between the strands of the third -hairpin (5 -- 6), and two cysteines from the N-terminus of helix H9 (Cys168 and Cys172). This arrangement of zinc-coordinating residues is typical of treble-clef zinc fingers. Other strictly conserved residues in this domain include Asp158 and Arg175. A…
123). In this study, Martinez-Contreras and her associates report the results of recent research that has provided additional evidence concerning the function of these proteins in precursor-messenger RNA (pre-mRNA) splicing (2007). The splicing repression can function in two discrete ways in heterogeneous nuclear RNP proteins; the first way is by antagonizing the recognition of splice sites directly and the second way is through interference with the binding of proteins that
Biochemistry Similarity of Glycolysis in Prokaryotes and Eukaryotes Glycolysis is reported as being a pathway that is practically universal for energy extraction that carbohydrates hold available and this is true for eukaryotes, prokaryotes as well as aerobes and anaerobes. (Essential Biochemistry, 2014, paraphrased) Only eukaryotes have mitochondria. Some prokaryotes are reported to be photosynthetic and to use "an electron transport chain to make ATP." (Essential Biochemistry, 2014, p. 1) It is believed that
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The next database used for the search is Medline. I began with the same search terms, celiac disease biochemistry, however it did not return any articles. Next, the search was expanded my search by simply searching celiac disease. This returned 544 articles. The first article is titled Celiac Disease and is from the National Library of Medicine. This article is clearly not useful. Next the search was expanded to celiac
student with a Bachelor of Science degree in Communications, minors in both Psychology and Sociology, and a desire to attend law school, my request to enter a Biochemistry program may be unusual. After all, people who focus on the hard sciences usually do so because of plans to work in a particular industry or to pursue additional field-specific education via Masters or PhD programs. Because I have no intention
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