For the temperature portion of the investigation (conducted first), eight test tubes were placed in four temperature controlled water baths ranging from water and crushed ice (2( C) to near boiling (98(). The other baths were kept at room temperature (23( C) and an intermediary between this and boiling (75( C). Test tubes were left in the baths for ten minutes, until their temperatures at equalized. Starting with the coldest bath, the amylase mixture was poured into the starch solution and briefly stirred. Leaving the test tube in the bath, a single drop was removed every ten seconds using a plastic pipette and placed into a well containing the iodine solution. When the color remained orange, the reaction had been completed, and the time (i.e. length of reaction) was noted. This same process was repeated with the other three sets of test tubes at the other temperatures, washing and replacing the iodine trays when needed.
To test for pH efficiency, different concentrations of the buffer solution were created at pH 3,5, 7, and 9. These were added one at a time to four separate test tubes of starch solution, and then the amylase solution was introduced. Again, drops of the combined solutions were transferred to wells on the iodine tray, and when the iodine solution remained orange the reaction was considered complete and the time noted.
Results
The results of the temperature portion of the experiment appeared fairly straightforward. At near freezing temperatures, the reaction took nearly eight minutes to complete. This time was cut in half at room temperature, and was diminished significantly again at 75( C. At 98( C, (near boiling), however, the reaction never took place. After ten minutes without change in the iodine solution, it was assumed that the amylase had been effectively destroyed by the high temperature and that no reaction was taking place.
7.7
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2 23 75
Temperature
In the pH experiment, the results were lightly more complex; rather than a simple increase in the efficiency of the reaction, a noticeable dip in the reaction time occurred in the neutral pH sample (pH 7), with effectiveness...
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