Substance in Green Tea Inhibits Inflammatory Breast Cancer Cells
Green Tea & Inflammatory Breast Cancer Cells
What is the overall hypothesis of this experiment?
Consumption of epigallocatechin-3 gallate (EGCG) in green tea will reduce the distinct growth and dissemination properties of Inflammatory Breast Cancer (IBC) cells in culture and on tumor growth in an orthotopic mouse model, as mwasured by reduced growth, invasive phenotype, and survival of SMU-149 and SUM-190 IBC cells, and the ability of the IBC cells to stimulate in vitro lymphangiogenesis.
In the Materials and Methods section of the paper, the authors discuss the cell culture and treatment conditions. What was the control group treated with in this section (make sure to look only at the Cell Culture and Treatment section of the paper)? Why?
The control group was treated with carrier dimethyl sulfoxide (DMSO) equivalent to the highest does of EGCG used, which is calculated at DMSO = 0 ?g / ml EGCG. The DMSO chemical may be used to dissolve other chemicals or drugs that are otherwise difficult to dissolve in water. This step makes it easier to test substances in animal experiments or in cells that are growing in Petri dishes. When DMSO is used in this way, the experimental...
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