The purified DNA is then prepared using a PCR like procedure that is described in detail by Innis and then can be automatically sequenced using standard methods (Hirashi). The resulting DNA sequence can then be entered into the NCBI database to search for a bacterial match. The database can be found at the following web address: http://blast.ncbi.nlm.nih.gov/Blast.cgi.
Results
Using PCR and DNA sequencing techniques it was determined that the bacteria isolated from a patient sample was Bartonella henselae. The results of the DNA sequencing can be seen in figure one. This is the output that was inputted into the NCBI database to search for a bacterial match. The top five results of the NCBI database search for the DNA sequence can be seen in table one. The results indicate that the bacterium was likely Bartonella henselae. Rochalimaea is an old genus name for Bartonella.
Discussion
PCR and DNA sequencing was successfully used to identify a bacterium that was isolated from a patient sample. The bacterium was identified to be Bartonella henselae. B. henselae is known to cause cat scratch disease and is transmitted to humans via a cat vector. Some common symptoms of cat scratch disease are swelling at the site of injury, fever and fatigue. It is generally not treated but in more severe cases antibiotics may be prescribed. In the future, the experiment could be repeated for other isolated colonies grown from the patient...
The experiment was designed to first extract DNA, take it through the steps of amplification and detachment, and then prepare it for analysis. The question was what type of bacteria was being sequenced. The steps used were: 1) Extraction of genomic DNA, 2) Amplification of genetic region, 3) Verification, 4) Clean PCR Products, 5) Quantify DNA concentration, 6) Cycle the sequence, and, 7) Precipitate cycle-sequenced products for analysis. In
Future Applications of Forensic DNA Analytical Methods Following the description of the structure of deoxyribonucleic acid (DNA) by Nobel Prize laureates Francis Crick and James Watson in 1953, a vast array of applications have emerged based on this structure. Indeed, scientists are identifying innovative ways to treat a host of human ailments based on this discovery, and future research will undoubtedly continue to identify additional applications for this information. Other
Tests are therefore conducted to examine if there is a presence of S. aureus that caused the illness and if the bacteria is or can be recognised as a potential source for food poisoning (Bennet & Lancette, 2001). One such test is known under the name of Direct Plate Count Method as it was illustrated by Bennet and Lancette, generally requiring a step-by-step procedure involving a. Equipment and materials,
Transcription is a process that genetic information on the DNA copies into RNA and the DNA acts as the template for the new molecules of RNA. Transcription process begins with the DNA double helix unwinding as the hydrogen bonds holding the opposing bases breaks and the DNA strands are uncoupled. The process occurs within the cytoplasm of a prokaryote and in the nucleus of eukaryotic cells. Transcription process consists
Molecular Basis Glanzmann Thrombasthenia An investigation of the molecular basis of Glanzmann Thrombasthenia using Polymerase Chain Reaction (PCR) The objective of this project is to investigate the molecular basis of Glanzmann Thrombasthenia (GT) using polymerase chain reaction. There have been many mutations discovered in GT patients over the years in many studies. Thus using PCR to genotype patients is one of the most effective ways of discerning the genetic basis of the
" (Volpicelli-Daley and Levey, 2003) Prior to visualization of the molecule of interest it is necessary to "fix and section the brain tissue. Double-labeling immunofluorescence is stated to detect "localization of a protein of interest as well as the distribution of the protein relative to another marker such as a neurochemical or organelle marker." (Volpicelli-Daley and Levey, 2003 Fluorescence imaging labeled tissue through use of confocal makes provision of "high-resolution analysis
Our semester plans gives you unlimited, unrestricted access to our entire library of resources —writing tools, guides, example essays, tutorials, class notes, and more.
Get Started Now