Plasmids
"Construction of the mobilizable plasmid pMV158GFP"
"Construction of the mobilizable plasmid pMV158GFP" is an article that describes the construction of a new, mobilizable plasmid, based on the pMV158 plasmid; but containing the gene which codes for green fluorescent protein (gfp) and is controlled by a maltose inducible promoter (Pm). The new pMV158GFP plasmid allows for bacteria which contain it to glow green under UV light, indicating whether or not the plasmid has been incorporated into, or mobilized, the Gram-positive bacteria. This new plasmid will allow for a better understanding of the processes and development of infectious bacteria in their natural environment.
Plasmids are circular, self-replicating pieces of DNA which are used to insert other pieces of DNA into cells and can be extremely useful in biological research. Nieto and Espinosa use one particular plasmid, pMV158, to create a new one which is designed for their specific needs. They intend to study the progress and development of infectious bacteria, but first needed to develop a mobilizable plasmid which could be used to mark specific ones. They did this by combining a well-known plasmid with new pieces of DNA which added a controllable marker to the plasmid. This marker was the gfp gene; a gene which could be induced into...
Scientific research and specifically cloning is protected as a first amendment right, coupled with the benefits available with this technology, and the unimaginable benefits that can be reaped in the future, cloning is the hope of the future, despite the worries of critics. References After Dolly: The Uses and Misuses of Human Cloning." The Futurist 40(4) Jul-Aug 2006: p. 62. InfoTrac database. Thomson-Gale. University of Phoenix, Phoenix, AZ. July 5, 2006
Legal costs might also haunt governments that allow cloning research. To prevent complications related to direct government investments in cloning research, legislation could open the door for privately-funded cloning research projects while at the same time banning federally- or state-funded research projects. However, most opponents of cloning cite the ethical costs involved in cloning legislation. Opponents of stem cell research sometimes "argue that permitting nuclear transplantation would open the door
and, that is, for how much longer should this experimentation be tolerated given the animal suffering involved and the deliberate creation of abominations of nature. Currently, many countries around the world have banned the use of reproductive, human cloning on ethical grounds, while allowing research to continue in the area of therapeutic cloning and reproductive cloning of animals. Of course, there are also countries that are permitting the development of
The virus genome covered by the capsid penetrates the host cell. Once inside, the virus is uncoated as the envelope and capsid are removed. Free of its covering, the viral genome (DNA or RNA) proceeds with biosynthesis. Newly assembled viral particles are released by budding. Components of viral envelopes (i.e., lipids, proteins, and carbohydrates) are obtained from the plasma or nuclear membrane as the viruses leave the cell. Budding
Transcription is a process that genetic information on the DNA copies into RNA and the DNA acts as the template for the new molecules of RNA. Transcription process begins with the DNA double helix unwinding as the hydrogen bonds holding the opposing bases breaks and the DNA strands are uncoupled. The process occurs within the cytoplasm of a prokaryote and in the nucleus of eukaryotic cells. Transcription process consists
Ian Wimut and Keith Campbell could effectively clone two sheeps named Megan and Morag in July 1995 from the differentiated embryo cells. (History of Cloning) Dolly originated on July 5, 1996 as the first organism ever to be cloned from adult cells. Following the announcements for creation of Dolly by Ian Wilmut, an extensive debate on human cloning ethics emerged and that led President Clinton to propose for a five-year
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