There was no further change observed until T=3 minutes where the solution began turning a lighter color. At T=4.5 it changed to amber and began to lighten. At T=8.5 it changed to clear and the starch was used up.
Room Temperature Tube
The second test tube was allowed to maintain a room temperature of 24 degrees Celsius the room temperature tube had an almost instant reaction at T=.5 minutes. By T=1 minute, the solution had lightened significantly. At T=1.5 minutes, the starch was degraded and the solution was clear. This was by far the fastest reaction.
80 Degrees Celsius, Hot Water Bath
The first hot water bath solution was brought and maintained at a temperature of 80 degrees. The theory is that this test tube should react even faster than the previous test tube because of the added heat. At T=1.5 minutes, the solution was black. At T=2.5 minutes, the solution changed to blue. After that point, the solution no longer reacted. The starch failed to fully degrade which meant that the enzyme failed to complete the process.
100 Degrees Celsius, Hot Water Bath
The second hot water bath solution was brought and maintained at a temperature of 100 degrees Celsius at T=.5, the solution was black. The solution was failing to change, so an additional drop was added at T=2.5. By T=3 the solution changed to purple and by T=5.5 the solution lightened to blue. The solution failed to further convert the starch from that point forward.
Discussion
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