Biofilm
In order to evaluate the validity of the hypothesis "catabolite control of S. epidermidis biofilm formation is indirectly regulated by CcpA-dependent of the TCA cycle," a laboratory study was performed and then documented in the article "CcpA coordinates central metabolism and biofilm formation in Staphylococcus epidermidis." Through their research, the authors of the article ultimately determined that CcpA is in fact a positive effecter of biofilm formation of S. epidermidis, along with icaADBC transcription and a repressor of TCA cycle activity.
The general profile of S. epidermidis shows that it is a pathogen that is opportunistic in nature, therefore primarily infecting patients who are immunocompromised. It is often a cause of infections for patients who receive implanted biomedical devices. This pathogen makes itself particularly difficult to treat in these situations due to the formation of a biofilm, which encapsulates the bacteria in an exopolysaccharide matrix. It is therefore valuable to discover a way in which to inhibit the formation of these biofilms in order to have a strong defense against the domination of this pathogen within a patient (Sadykov 2011).
It is a known that the formation of these biofilms, as well as the synthesis of the exopolysaccharides synthesis, is significantly influenced by the availability of nutrients and the environmental conditions. Of particular note pertaining to this study, the formation of the biofilm is enhanced while growing in media that contains glucose. What this fact would suggest is a carbon catabolite-responsive regulator activates the genes that are essential to the formation of biofilm, and/or it represses the genes that inhibit the formation of the biofilm (Sadykov 2011).
The authors of this article used many methods to conduct their examinations of the role of the CcpA in the ability of the pathogen to create a biofilm. This included constructing a S. epidermidis mutant, which contained an inactive CcpA. They then observed the effects that it had upon growth, biofilm formation, and virulence. Based on the data, the...
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